Correlation between Certain Laboratory Stress Tests and Fertility of Frozen Bovine Spermatozoa

The Viability and Fertility of Bovine Spermatozoa

Effect of thawing procedures on fertility of bovine spermatozoa frozen in .25-ml straws.

The influence of thawing procedures on fertility of semen in .25-ml Continental 3 straws was investigated in a series of eight trials utilizing 1,634 cows. The following pregnancy rates were obtained: Trial 1, 38.6 vs 23.0% (P<.05) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 2, 29.7 vs 22.2% (P>.10) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 3, 82.8 vs 61.6% (P<...

Fertility of ram spermatozoa pellet-frozen in zwitterion-buffered diluents.

The fertility (positive ultrasound scanning 60-75 days after insemination) of ram spermatozoa frozen in Tris- or zwitterion/glucose/egg yolk/glycerol-based diluents was examined during the natural breeding season (autumn). In Experiment 1 Tris-citrate, high glucose and Hepes-NaOH diluents were used and ewes (n = 266) were inseminated (induced oestrus) with 20 (uterus) or 180 (cervix) million mo...

Reaction of fresh and frozen bull spermatozoa incubated with fresh and frozen bovine oviduct epithelial cells.

The hypothesis was tested that frozen bovine oviduct epithelial cells (BOEC) could provide a physiologic and standardized system for studying motility and acrosomal changes of fresh and frozen-thawed sperm. The BOEC were collected by lavage from cows and monolayers prepared from both fresh and frozen-thawed cells. Fresh and frozen semen processed from five bulls was extended in a modified Tyrod...

Laboratory tests for oxidative stress

INTRODUCTION Oxidative stress (OS) is considered a significant contributor to male infertility. A number of laboratory techniques have been developed to evaluate oxidative stress in the semen. We review these tests and their current use. METHODS A literature review was performed utilizing the PubMed search engine for articles studying OS etiology and impact on male fertility, and the laborato...